August 5, 2021

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What Are The Differences Between Rabbit And Mouse Antibodies?

2 min read

Antibodies are the most commonly used tools in biological research. They are used in various applications such as Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), and enzyme-linked immunosorbent assays (ELISA).

You can also visit https://www.bosterbio.com/featured-products to know more about rabbit and mouse antibodies. The two most common hosts for research antibodies are rabbits and mice, but what is the difference between rabbit and mouse antibodies? Which antibody is best for your test? 

Antigen recognition and multiple antibody reservoirs

Rabbits as hosts have a high success rate with more antigens than mice. In mice, antigens such as small molecules and peptides are often non-immunogenic, while rabbits produce the desired antibodies against antigens, including small molecules, peptides, and post-translational modification sites (PTMs).

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The rabbit antiserum produced consisted of a greater variety of antibodies than the mouse antiserum.

In addition, antibodies produced in rabbits usually recognize more protein antigen epitopes than antibodies in mice because rabbits have less immunodominance.

Affinity and specificity

In general, rabbit antibodies have better affinity and specificity than mouse antibodies. Rabbit antibodies are highly specific antibodies that can bind to proteins in the picomolar range, while mouse antibodies recognize proteins in the nanomolar range with medium/high specificity.

Application

Antibodies made in mice are generally suitable for general applications such as WB, ELISA, flow cytometry, and IP. However, they tend not to produce satisfactory results in staining applications such as immunohistochemistry (IHC) and immunocytochemistry (ICC).

In comparison, rabbit antibodies performed better in this assay, especially when used on tissue samples from mice.

Vaccination duration

Mouse antibodies require a shorter immunization time due to the size of the host. Rats usually need one and a half months of immunization, rabbits two to three months.